PINEHURST, N.C., May 15, 2015 /PRNewswire-iReach/ — Assisi Animal Health is the developer of the Assisi Loop TM, an effective non-pharmaceutical and anti-inflammatory device (NPAID). The Loop can help cure chronic wounds and treat a range of debilitating medical conditions including Wobblers, IVDD and lumbar stenosis. Recently, researchers developed artificial equine skin. Assisi Animal Health discusses how this development affects the veterinary equine industry.
While scientists have already grown tissue-engineered skin for dogs, cats, humans and mice, they found it difficult to grow skin in a laboratory for horses. The basic building blocks of equine skin cells, called primary keratinocytes, do not survive long in laboratory cultures.
However, a group of researchers in Spain recently perfected a method to grow keratinocyte cultures, which are now able to survive long enough to grow into healthy, tissue-engineered skin for horses. Artificial skin helps burn victims and those with severe skin wounds. Despite this being a new approach for regenerating tissue in horses, experts say it offers a safe and useful method of healing equine burns and other deep wounds.
Anna Puigdemont, Ph.D., of the Department of Pharmacology, Therapeutics and Toxicology at the University of Barcelona, describes how her group of researchers overcame the lack of longevity in keratinocytes. They acquired skin biopsies from horses that had died, and then removed the blood vessels and fat before digesting them in a special collagenase medium.
The enzyme collagenase disrupts peptide bonds found in collagen, the primary component in connective tissues of the skin. With careful management and frequent culture medium changes at exact intervals, they successfully grew biological skin within two weeks from their laboratories.
After 14 days, the skin is ready for use on wounds or further laboratory tests on equine skin. Puigdemont’s team verified the composition of their skin cultures microscopically, and their findings showed the tissue-engineered skin to be fully compatible with the original skin biopsies. It contained a correctly structured dermis and epidermis, the two main layers found in natural skin.
Tissue-engineered skin does not contain hair follicles or blood vessels. This means that while it is effective for closing wounds, its final appearance mimics a bald scar. However, it poses a more promising alternative to transplanting synthetic skin or taking natural skin from elsewhere on the horse’s body, as both methods still face challenges.
While there is no photo evidence of the equine healing process yet, in other animals, the lesion decreases enormously after application of tissue-engineered skin, and the scar becomes an unimportant issue in light of the significant healing it offers. Currently, the team grows equine tissue for veterinarians in need of replacement skin to help their patients recover from burns and other skin injuries.
Assisi Animal Health’s clinical solutions complete the Circle of Care TM — the collaboration of veterinarians and owners in animal health and healing. Our company helps veterinary professionals and owners improve the quality of life for companion animals using the Assisi Loop, the non-invasive, non-pharmaceutical healing device that is based on the same FDA-cleared technology used on humans. The device uses low-level pulses of electromagnetic energy to reduce pain and swelling, and to enhance recovery.
The Assisi Loop. Proven Science. Positive Results. TM
For more information, visit our website: www.assisianimalhealth.com.
Media Contact: Kaysie Dannemiller, Assisi Animal Health, 1-866-830-7342, firstname.lastname@example.org
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SOURCE Assisi Animal Health